Human Bone Marrow Stromal Cells

Human bone marrow stromal cells derived in acf medium catalog 70071 using the mesencult acf culture kit catalog 05449 differentiate to a adipocytes oil red o staining b chondrocytes alcian blue and nuclear fast red staining and c osteoblasts alizarin red s staining.

Human bone marrow stromal cells.

1 2 due to their potential for differentiation into osteocytes chondrocytes myocytes and adipocytes mscs have emerged as a promising tool for clinical applications such as tissue engineering and cell and gene therapy. Human bone marrow stromal cells suppress t lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Human bone marrow stromal cells mscs more recently referred to as mesenchymal stem cells are capable of differentiating along multiple mesenchymal lineages in addition to supporting hematopoiesis. In addition to their anti inflammatory and immunomodulatory characteristics lee et al 2009a.

Additionally the bone marrow stroma contains cells with a stem cell like character that allows them to differentiate into bone cartilage adipocytes and hematopoietic supporting tissues. Casiraghi et al 2010 they have the capacity to differentiate directly along skeletal and other mesenchymal lineages pittenger et al 1999. Our ultimate goal of in vitro derivation of schwann cells scs from adult bone marrow stromal cells bmscs is such that they may be used autologously to assist post traumatic nerve regeneration. Human bone marrow stromal cells hbmscs are very rare skeletal stem cells and important constituents of the hematopoietic microenvironment.

Primary hbmscs are traditionally identified retrospectively based on their potential to adhere to plastic and to form fibroblastic colonies cfu f in vitro. Human bone marrow stromal cells hbmscs have therapeutic potential in cell transplantation and tissue engineering. Directed differentiation of human bone marrow stromal cells to fate committed schwann cells. Augello and de bari 2010.

Cd2 t lymphocytes obtained from either the donor of bone marrow stromal cells bmscs or a third party were cultured in mixed lymphocyte reactions mlrs with either allogeneic dendritic cells dcs or peripheral blood. Adult bone marrow also contains stromal stem cells which contribute to the regeneration of several tissues such as bone cartilage muscle ligament tendon adipose tissue and marrow stroma 9 human bmscs have been identified and characterized and it has been shown that they support hematopoietic progenitors and secrete a number of hematopoietic cytokines both constitutively and in response. The bone marrow stroma consists of a heterogeneous population of cells that provide the structural and physiological support for hematopoietic cells. Human bone marrow stromal cells hbmsc.

The terms multi lineage progenitor cells and marrow stromal cells msc have recently been defined cells isolated by adherence to plastic during ex vivo culture of bone marrow are termed.